|
KMID : 1101320130450020048
|
|
Korean Journal of Clinical Laboratory Science
2013 Volume.45 No. 2 p.48 ~ p.53
|
|
|
Species Identification of Nontuberculous Mycobacteria (NTM) by PCR-Restriction Fragment Length Polymorphism (PRA) of the rpoB Gene from Three Hospitals of Busan-Kyeongnam Area
|
|
Choi Sung-Ran
Kang Min-Jung
Park Gyu-Hwan
Kim Da-Hye
Jeong Da-Woon
Seo Eun-Hye
Lee Hyang-Min
Park Hyun-Kyung
Jeong Jin-Yee
Lee Jung-Min
Jeong Soo-Young
Lee Jun-Young
Cho Eun-Jin
Jekal Suk
Kim Chung-Hwan
|
|
|
Abstract
|
|
|
|
Recently, the isolation rate of nontuberculous mycobacteria (NTM) in clinical laboratories and the incidence of NTM infections are on the increase in Korea, but there have been only a few studies that reveal the general aspect of NTM isolation or species distribution. Therefore, this study was performed to examine the species identification by PCR-restriction fragment length polymorphism analysis (PRA, PCR-RFLP), and the clinical significance of mycobacterial cultures. PRA was used during the novel region of the rpoB gene and was developed for rapid and precise identification of mycobacteria to the species level. From January 2012 to April 2012, we examined pre-identified nontuberculous mycobacteria (60 species in 3 hospital of Busan-Kyeongnam area). We confirmed 4 (6.6%) Mycobacterium tuberculosis (MTB) and 56 (93.4%) NTM from 60 pre-identified NTM species by multiplex PCR (MolecuTech MTB-IDR V3, YD Diagnostics, Korea) and PRA (Myco-ID, YD Diagnostics, Korea). The distribution of 56 NTM species were M. intracellulare type I 15 (26.7%), M. avium 14 (25%), M. abscessus 11 (19.5%), M. kansasii type I 3 (5.4%), M. pulveris 2 (3.6%), M. intracellulare type, M. chelonae, M. kansasii type V, M. gallinarum, M. wolinskyi. Respectively, 1 (1.8%) and 6 (10.7%) species were not identified.
|
|
|
KEYWORD
|
|
|
NTM, MTB, PCR, PRA (PCR-RFLP)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|